| Ghrelin

Ghrelin (GHRL) is a hormone produced by enteroendocrine cells in the gastrointestinal tract, particularly the stomach, which increases food intake. During hunger, blood ghrelin levels peak before meals and return to lower levels afterward. Ghrelin may help prepare for food intake by increasing gastrointestinal motility and gastric acid secretion. Ghrelin activates cells in the anterior pituitary and hypothalamic arcuate nucleus, including neuropeptide Y neurons that initiate appetite. Ghrelin stimulates brain structures with specific receptors—the growth hormone secretin receptor 1A (GHSR-1A). The mRNA produced by the GHRL gene contains four exons. Five products are generated: the first is pro-ghrelin, consisting of 117 amino acids. It is homologous to promotilin; both are members of the motilin family.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	400	200	100	50	25	12.5	6.25	0
OD	0.216	0.297	0.417	0.669	0.955	1.405	1.79	1.865

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Ghrelin spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101
Cell Culture Supernates(n=8)	97-108	105

 

| Linearity

Samples were spiked with high concentrations of Ghrelin and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-104	87-108	105-115
	Average (%)	93	98	110